Table 1.

Relative amounts of modified nucleotides in wild type tRNA^[Ser]Sec and mutants and the SB-217452 treated tRNA^[Ser]Seca

		U-labeled			A-labeled
		pmcm5Ub	pmcm5Umb	pψc	pm1Ad	pi6Ae
GCCA[WT]	I	±	+	+	+	−
GCCA[WT]	II	++	±	+	±	+
ACCA	I	++	−	+	+	−
ACCA	II	++	−	+	±	+
CCCA	I	++	−	+	+	−
CCCA	II	++	−	+	±	+
UCCA	I	++	−	+	+	−
UCCA	II	++	−	+	±	+
GCCA[WT]	I	±	+	+	+	−
GCCA[WT]	II	++	±	+	+	+
GCCA[WT] +SB−217452	I	++	−	+	+	−
GCCA[WT] +SB−217452	II	++	−	+	+	+

^aThe amount of each modified nucleotide was calculated using a PhosphorImager and is relative to the amount of pU or pA.

^b++: 15–25 %; +: 5–15 %; ±: 1–5 %; −: < 1 % of pU.

^c+: 15–25 % of pU.

^d+: 10–20 %; ±: 1–10 % of pA.

^e+: 35–55 %; −: < 1 % of pA.