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. Author manuscript; available in PMC: 2011 Jun 28.
Published in final edited form as: J Invest Dermatol. 2007 Oct 18;128(4):833–845. doi: 10.1038/sj.jid.5701103

Figure 3. Restoration of one or two tyrosine residues in the PKCδ-Δ5 mutant restores hINV promoter activity.

Figure 3

(a) Expression of vector-delivered proteins. Keratinocytes were transfected with 1µg of empty plasmid vector (EV) or plasmid encoding the indicated protein. After 48 hours, the cells were harvested and total cell extracts were prepared for electrophoresis and immunodetection of vector-delivered PKCδ. (b) Twenty-five percent confluent keratinocytes were transfected with 1µg of pINV-241 promoter reporter plasmid + 1 µg of empty expression vector (EV) or the indicated PKCδ-encoding plasmid. At 48 hours post-transfection, the cells were harvested and lysed for luciferase activity assay. The results show the mean±SEM, n = 4.