Table 1.
Details of methodology used in genotyping
| SNP ID | Location | Primer sequence 5’–3’ | Tm (ºC) | Enzyme | RFLP pattern |
|---|---|---|---|---|---|
| Ex06+139C/T rs1202168 | chr7:87033898 | AGGTTTCATTTTGGTGCCTG | 61.5 | SspI | 299* 275, 24# |
| GAACAAAAGGATGCACACGACA | |||||
| Ex 12 C1236T rs1128503 | chr7:87017537 | TACCTGTGTCTGTGAATTGCC | 59.3 | HaeIII | 269, 62, 35* |
| CCTGACTCACCACACCAATG | 269, 97# | ||||
| Ex 17-76T/A rs1922242 | chr7:87011603 | TTTGTCAACATTTTTTTGAAGC | 69.2 | ApoI | 231, 84* 120, 111, 84# |
| TATTATTGCAAATGCTGGTTGC | |||||
| Ex 21G2677T/A rs2032582 | chr7:86998554 | TGCAGGCTATAGGTTCCAGG | 67.9 | BanI | 198, 26* |
| TTTAGTTTGACTCACCTTCCC | 224# | ||||
| Ex26 C3435T rs1045642 | chr7:86976581 | TGTTTCAGCTGCTTGATGG | 59.4 | Sau3AI | 158, 39* |
| AAGGCATGTATGTTGGCCTC | 197# |
*
wild type
#
mutant