Histogram of CT numbers with the corresponding 4-tissue (in blue) and 8-tissue (in red) material assignment of the microCT data set. Dashed lines define air, lung, adipose, muscle, spongiosa, ribs (2,6), cranium, and cortical bone as used in the 8-tissue segmentation model. Adipose and muscle are combined into muscle and spongiosa, ribs (2,6), cranium, and cortical bone are combined into cortical bone in the 4-tissue segmentation model.