Fig. 2.

GATA4 is localized to osteoblasts in vivo and is induced by E2. A, Femurs from wild-type (WT) and ERαKO mice were snap-frozen and embedded in OCT. The 10-μm sections were stained with RUNX2 and GATA4. Images are of trabecular bone near the growth plate. B, Calvariae from neonatal d-2 mice were grown as organ cultures for 7 d and then treated with vehicle control [ethanol (EtOH)] or 10 nm E2 for 24 h. Calvariae were fixed and paraffin embedded. Serial sections were stained with hematoxylin and eosin (H&E) or RUNX2 and GATA4. Arrows indicate RUNX2 and GATA4 colocalization. DAPI, 4′,6-Diamidino-2-phenylindole.