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JAK2 is tyrosyl phosphorylated in M2 and A7 cells overexpressing PRLR in response to PRL. Human PRLR was overexpressed in M2 and A7 cells. The cells were deprived of serum and treated with or without 400 ng/ml PRL for 15 min. JAK2 was immunoprecipitated with αJAK2. The immunoprecipitates and whole-cell lysates were immunoblotted with indicated antibodies. Each experiment was performed at least three times with similar results. IP, Immunoprecipitation.
