Table 1.
Sweet potato chlorotic stunt virus isolates used in this study and sequence accession numbers. Strain was determined by serology using antiserum MAb mix 1 and mix 2 (Gutierrez et al., 2003) for isolates 1–19 and by sequence analysis of hsp70h for isolates 20 and 21. Presence or absence of p22 was determined by PCR-RFLP as described in materials and methods and confirmed by sequencing in selected cases.
| SPCSV Isolate | Origin | Strain | p22 | RNA1 3′-UTR | RNA2 3′-UTR | RNA2 5′-UTR |
|---|---|---|---|---|---|---|
| (1) m2-47 | Cañete, Peru | EA | NO | HQ291259 | HQ291260 | HQ291260 |
| (2) Set2 | San Ramon, Peru | EA | NO | |||
| (3) Set4 | San Ramon, Peru | EA | NO | |||
| (4) Pelotas | Pelotas, Brazil | WA | NO | |||
| (5) EL | El Indio, Argentina | WA | NO | HQ847524 | ||
| (6) Arg | Cordoba, Argentina | WA | NO | HQ847529 | HQ847534 | |
| (7) Africa10 | Kenya | EA | NO | HQ847523 | ||
| (8) SR | San Ramon, Peru | EA | NO | |||
| (9) Fe | Ferreñafe, Peru | EA | NO | |||
| (10) Fio | Cañete, Peru | EA | NO | HQ847528 | HQ847533 | HQ847522 |
| (11) KmtMil | Cañete, Peru | EA | NO | |||
| (11) m2-41 | Cañete, Peru | EA | NO | |||
| (12) m2-44 | Cañete, Peru | EA | NO | |||
| (13) m2-63 | Cañete, Peru | EA | NO | |||
| (14) C14 | Cañete, Peru | EA | NO | |||
| (15) C18 | Cañete, Peru | EA | NO | |||
| (16) C21 | Cañete, Peru | EA | NO | |||
| (17) Hua | Huaral, Peru | EA | NO | HQ847520 | ||
| (18) Chi4 | Chimbote, Peru | EA | NO | HQ847527 | HQ847532 | HQ847521 |
| (19) Chi2 | Chimbote, Peru | EA | NO | |||
| (20) KSR675 | Kisoro, Uganda | EA | YES | HQ847530 | HQ847535 | HQ847525 |
| (21) Bitambi | Masaka, Uganda | EA | YES | HQ847531 | HQ847536 | HQ847526 |