Nested PCR to detect unique Vα24-JαQ TCR sequences in skin biopsy specimens. RNA was extracted from an NKT cell line, Jurkat cells, and foreskin KC (FSKC), cDNA was synthesized, subjected to the nested PCR, and run on an agarose gel as described in Methods. (a) Control lane (Ctrl) represents no cDNA added to nested PCR. (b) This same nested PCR was applied to normal skin (NORM), psoriasis (PSOR) and five cases of ACD (CD1–5). (c) Nested PCR from same specimens was subjected to real-time PCR amplification, with SYBR green as the fluorochrome (*P<0.05; **P<0.001; ***P<0.01, compared to normal skin, ANOVA).