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. Author manuscript; available in PMC: 2011 Jun 28.
Published in final edited form as: J Mol Biol. 2009 Feb 21;387(5):1199–1210. doi: 10.1016/j.jmb.2009.02.037

Fig. 6. Cartoon of the proposed reaction schemes.

Fig. 6

Distortions of the carboxyl, cyano, iodo and azido groups play an important role in the reaction catalyzed by ODCase. “P-Rib” abbreviates ribose-5′-monophosphate. (a) The reaction from OMP to UMP catalyzed by native ODCase. (b) Covalent bond formation between the mutant ODCase and 6-CN-UMP. (c) The conversion of 6-CN-UMP to BMP catalyzed by native ODCase. (d) Covalent bond formation between the native ODCase and 6-I-UMP or 6-N3-UMP. (e) The conversion of 6-I-UMP to BMP by K72A MtODCase. Distortion of the bond linking the pyrimidine ring to the C6-substituent is conserved in all these reactions, the physiologically relevant decarboxylation as well as the nucleophilic mechanisms.