Table 1. Transcriptome feature of three developmental stages from Clonorchis sinensis.

	Adults	Metacercariae	Eggs	All
Total sequence reads	30,144	20,256	10,368	60,768
Total analyzed reads +	27,070	15,872	9,803	52,745
Average EST size (bp after trimming)	528	524	569	522
Total number of assembled sequences	7,779	5,398	2,660	12,830‡
Contigs	3,921	2,728	1,523	7,184
Singlets	3,858	2,670	1,137	5,646
Average contigs size (bp)	720	671	758	724
Total known genes *	3,993	2,718	1,630	6,413
Unigene number	3,488	2,427	1,504	5,269
Unigenes matched to S. mansoni or S. japonicum	1,674(48%)	1,138(47%)	868(58%)	2,356(45%)
Unigenes matched to other organisms	1,814(52%)	1,289(53%)	636(42%)	2,913(55%)
Total unknown genes	3,786	2,680	1,030	6,417

+: : Total analyzed reads were determined using the stringent quality filtering and contained reads with a minimum of 100 bases and a phred quality≥20.

*: Total known genes were determined by homology ≥25% and length of minimum exact match ≥30 amino acids by using BLASTX homology search.

‡: : Each stage (column) was generated from its own dataset. Independently, data of all stage were assembled from the combination of adults, metacercariae and eggs.