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. 2011 Jun 28;6(6):e21442. doi: 10.1371/journal.pone.0021442

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Ginet et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) Western blot assay of purified and immobilized Opd. The purified protein deposits are 117, 58, 29, 15 and 7 ng, from left to right. The magnetosome membrane was dissolved in 4% SDS and two deposits of solubilized membrane proteins were made (15 and 10 µl). Expected molecular weights for Opd and MamC-Opd are 34.6 and 49.6 kDa respectively. (b) Calibration curve for Opd quantification obtained with GeneTools image analysis software from Syngene. The computed Opd quantities in the solubilized magnetosome membrane proteins are 17.3 and 9.6 ng for the 15 and 10 µl of solubilized magnetosome membrane protein deposits (from left to right).