Figure 7. The effect of resealing erythrocyte ghosts in the presence of ankBDn and its mutants.

Hemoglobin (∼1 mg/ml) or bacterially expressed and purified ankBDn and its mutants, CLSC and SSSA (∼1 mg/ml), were present in the resealing buffer. Resealed erythrocyte ghosts were labeled with DID. Ghosts loaded with wild-type ankBDn show the most extensive shape change while those loaded with SSSA mutant resemble control ghosts loaded with hemoglobin. For details see Materials and Methods. Scale bars: 10 µm.