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. 2011 Jun 28;6(6):e21538. doi: 10.1371/journal.pone.0021538

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Wolny et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Overexpression of GFP-conjugated constructs; B) Immunofluorescent staining with anti-Na⁺/K⁺-ATPase antibodies; C) Overlay. Distribution pattern of Na⁺/K⁺-ATPase is changed in cells transiently overexpressing ankBDn and CLSC constructs. This is especially visible in cells overexpressing ankBDn, where GFP constructs colocalize with Na⁺/K+-ATPase. GFP plasmid was used as control. Scale bars: 20 µm.