Table 1.
Proliferation yield and phenotype analysis of lymphocytes expanded from metastatic lymph nodes of eight patients with melanoma
| Patients | Initial cell number | Final cell number | Fold expansion | CD4 a | CD8 a | CD8+/IFNγ+ TIL | |||
|---|---|---|---|---|---|---|---|---|---|
| (×106) | (×106) | % | Nb (106) | % | Nb (106) | % | Nb (106) | ||
| 01 (plates) | 0.39 | 794 (±501) | 2035 (±1287) | 56 (±8) | 439 (±257) | 43 (±9) | 342 (±257) | 2 (±1) | 5 (±2) |
| 01 (bag) | 0.39 | 1128 (±445) | 2892 (±1141) | 11 (±2) | 123 (±35) | 85 (±2) | 953 (±366) | 14 (±1) | 129 (±45) |
| 02 (plates) | 0.39 | 982 (±50) | 2518 (±128) | 80 | 797 (±49) | 19 (±4) | 191 (±54) | 2 (±1) | 3 (±1) |
| 02 (bag) | 0.39 | 1215 (±153) | 3115 (±393) | 17 (±6) | 207 (±50) | 80 (±3) | 981 (±166) | 5 (±1) | 45 (±14) |
| 03 (plates) | 0.39 | 1698 (±182) | 4352 (±466) | 5 (±1) | 85 (±19) | 95 (±1) | 1612 (±172) | 15 (±8) | 241 (±106) |
| 03 (bag) | 0.39 | 773 (±366) | 1984 (±938) | 1 (±0) | 6 (±4) | 99 (±0) | 766 (±362) | 15 (±5) | 107 (±31) |
| 04 (plates) | 0.39 | 895 (±217) | 2296 (±557) | 76 (±2) | 676 (±158) | 21 (±2) | 192 (±55) | 1 (±1) | 2 (±0) |
| 04 (bag) | 0.39 | 600 (±240) | 1539 (±615) | 61 (±18) | 387 (±257) | 33 (±16) | 180 (±94) | 2 (±1) | 3 (±2) |
| 05 (plates) | 0.39 | 989 (±180) | 2537 (±463) | 24 (±4) | 236 (±66) | 75 (±4) | 741 (±132) | 6 (±1) | 46 (±9) |
| 05 (bag) | 0.39 | 969 (±88) | 2484 (±225) | 8 (±3) | 76 (±17) | 90 (±2) | 870 (±100) | 12 (±4) | 107 (±44) |
| 06 (plates) | 0.39 | 910 (±199) | 2334 (±509) | 42 (±4) | 387 (±119) | 60 (±7) | 533 (±60) | 5 (±1) | 26 (±3) |
| 06 (bag) | 0.39 | 1323 (±152) | 3393 (±389) | 10 (±5) | 132 (±71) | 91 (±5) | 1205 (±162) | 23 (±2) | 281 (±58) |
| 07 (plates) | 0.39 | 1126 (±199) | 2889 (±509) | 24 (±5) | 274 (±73) | 75 (±2) | 851 (±174) | 16 (±4) | 132 (±27) |
| 07 (bag) (plates)) | 0.39 | 795 (±174) | 2039 (±446) | 6 (±1) | 46 (±18) | 93 (±1) | 737 (±162) | 26 (±8) | 184 (±29) |
| 08 (plates) | 0.39 | 772 (±123) | 1982 (±316) | 71 (±12) | 552 (±142) | 31 (±13) | 234 (±81) | 2 (±1) | 4 (±1) |
| 08 (bag) | 0.39 | 571 (±163) | 1465 (±417) | 46 (±17) | 281 (±182) | 54 (±14) | 296 (±24) | 5 (±3) | 14 (±9) |
For each patients, 3 or 4 independent cell expansions were conducted in parallel in bags and in plates and analysed at day 20. Values are the means obtained from these independent cell amplifications. Bracketed values show the standard deviation.
a, gated on lived CD3 + cells.