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. 2011 Jun 28;6(6):e21599. doi: 10.1371/journal.pone.0021599

Figure 7. GPR54 associates with EGFR.

Figure 7

(A) GPR54 co-immunoprecipitates EGFR in HEK 293 cells expressing FLAG-GPR54 and EGFR-GFP in the presence or absence of Kp-10 (100 nM). Representative blot of three independent experiments shown. (B) HEK 293 cells expressing FLAG-GPR54 and EGFR-GFP were incubated in the absence (0 min, i) or presence of Kp-10 (10 nM, ii) or EGF (10 ng/mL, iii) for 15 min. After 15 minutes of Kp-10 stimulation (ii), FLAG-GPR54 and EGFR-GFP partially co-localized in endocytotic vesicles (yellow). Similar results were obtained upon treatment of cells with 10 ng/mL EGF for 15 minutes (iii). Representative blot of three independent experiments shown. Scale bar, 10 µm. C) MDA-MB-231 cells stably expressing FLAG-GPR54 and EGFR-EGFP were incubated in the absence (0 min, i) or presence of Kp-10 (10 nM) for 15 min (ii). Representative confocal images of five experiments shown here. Scale bar, 10 µm.