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. 2011 Jun 28;6(6):e21520. doi: 10.1371/journal.pone.0021520

Figure 6. ERK1/2 activation by α1A-AR is independent of Gq/PLC/PKC pathway.

Figure 6

(A, B) Inhibition of phospholipase C (PLC) by U73122 affected ERK1/2 activation by α1B-AR but not α1A-AR. HEK-293A–α1A-AR or –α1B-AR cells were pre-incubated with 1 µM U73122 or prazosin or not for 30 min. Then cells were treated with 10 µM PE for 20 min. PKC and ERK1/2 activity was measured by western blot analysis. (C, D) Inhibition of PKC by Ro 31–8220 affected ERK1/2 activation by α1B-AR but not α1A-AR. HEK-293A–α1A-AR or –α1B-AR cells were pre-incubated with 1 µM Ro 31–8220 or prazosin or not for 30 min, then were treated with 10 µM PE for 20 min. PKC and ERK1/2 activity were measured by western blot analysis.