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. 2011 Jun 28;6(6):e21673. doi: 10.1371/journal.pone.0021673

Figure 1. Capacity of H27 and P104 M. avium strains to grow in macrophages and 7H9 broth.

Figure 1

Bacterial growth in the broth within 24 days was evaluated by spectrophotometry (A). Bone marrow cells were obtained from C57BL/6 mice and differentiated to macrophages in vitro. The resulted cells were infected at a MOI 1∶1 bacteria/macrophage, incubated for 3 h at 37°C, washed, tested for the mycobacterial uptake, and cultivated for additional 6 days. The resulted cells were lysed and CFU numbers (B) were quantified as indicated in Material and methods. The percentage of macrophages with internalized mycobacteria in the culture was quantified 3 h after infection by Ziehl-Neelsen method (C). The data obtained in three independent experiments are presented as mean ± SD of samples in triplicate. Asterisk represent statistical significance (*p<0.05).