Table 1. Nucleotide sequences of primers used in the RT-PCR.
Primer sequence (5′-3′) | ||
Cytokine | Forward | Reverse |
IL-12p35 | CCACTGGAACTACACAAGAACG | GCACAGGGTCATCATCAAAG |
IL-17 | AACATGAGTCCAGGGAGAGC | GCTGAGCTTTGAGGGATGAT |
TNF-α | ATGGCCTCCCTCTCATCAGT | CACTTGGTGGTTTGCTACGA |
iNOS | TCAAGTGGCATAGATGTGGAAG | TGGGTCCTCTGGTCAAACTC |
IL-10 | TCAGCCAGGTGAAGACTTTCT | TCATTTCCGATAAGGCTTGG |
IL-4 | GCAACGAAGAACACCACAGA | CTGCAGCTCCATGAGAACAC |
IFN-γ | TCAAGTGGCATAGATGTGGAAG | TGGCTCTGCAGGATTTTCAT |
β-actin a | GACGGCCAGGTCATCACTAT | ATGCCACAGGATTCCATACC |
Housekeeping reporter gene.