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. 2011 Jun 28;6(6):e21673. doi: 10.1371/journal.pone.0021673

Table 1. Nucleotide sequences of primers used in the RT-PCR.

Primer sequence (5′-3′)
Cytokine Forward Reverse
IL-12p35 CCACTGGAACTACACAAGAACG GCACAGGGTCATCATCAAAG
IL-17 AACATGAGTCCAGGGAGAGC GCTGAGCTTTGAGGGATGAT
TNF-α ATGGCCTCCCTCTCATCAGT CACTTGGTGGTTTGCTACGA
iNOS TCAAGTGGCATAGATGTGGAAG TGGGTCCTCTGGTCAAACTC
IL-10 TCAGCCAGGTGAAGACTTTCT TCATTTCCGATAAGGCTTGG
IL-4 GCAACGAAGAACACCACAGA CTGCAGCTCCATGAGAACAC
IFN-γ TCAAGTGGCATAGATGTGGAAG TGGCTCTGCAGGATTTTCAT
β-actin a GACGGCCAGGTCATCACTAT ATGCCACAGGATTCCATACC
a

Housekeeping reporter gene.