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. 2011 Jun 28;6(6):e21674. doi: 10.1371/journal.pone.0021674

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This is an open-access article free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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PBMC cultures from eight M. bovis-BCG-vaccinated calves were treated with 100 ng/ml LPS, 5 µg/ml PWM, or 10 µg/ml of M. bovis PPD or received no treatment (CTRL) as indicated for 24 h. The amount of 1α-OHase (A), VDR (B), iNOS (C), RANTES (D), IFN-γ (E), IL-17A (F), and IL-17F (G) mRNA was determined by quantitative real-time RT-PCR and was normalized to the amount of RPS9 mRNA in each sample. Expression of each gene is relative to non-stimulated cultures. Error bars represent SE. *** Mean is different from non-stimulated PBMC; P<0.001.