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. 2011 Jun 28;6(6):e21674. doi: 10.1371/journal.pone.0021674

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This is an open-access article free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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PBMC cultures from eight M. bovis-BCG-vaccinated calves were treated with 0 or 10 µg/ml of M. bovis purified protein derivative (PPD), 100 ng/ml of 25(OH)D₃, and 4 ng/ml 1,25(OH)₂D₃ as indicated for 24 h. The amount 24-OHase (A), iNOS (B), RANTES (C), IFN-γ (D), IL17A (E), and IL-17F (F) mRNA was determined by quantitative real-time RT-PCR. Each gene was normalized to the amount of RPS9 mRNA in each sample. Expression of each gene is relative the expression of that gene in non-stimulated cultures. Error bars represent SE. * Means are different; P<0.05.