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. 2011 Jun 28;6(6):e21657. doi: 10.1371/journal.pone.0021657

Figure 3. Experimental clonal evolution.

Figure 3

A. Schematic of the Lovo single cell cloning, first in culture and then as xenografts, simulating a clonal evolution bottleneck. B. Hitchhiking diversity decreases and then increases in culture and the xenografts after single cell cloning. (X's represent independent single cell clones in culture, and O's represent PWD averages among tags isolated from 5 to 6 small xenograft fragments) The LOC tag has a higher error rate compared to the BGN tag [12], and more quickly restores the diversity seen in polyclonal populations. C. Comparisons between fragments demonstrate intergland PWDs are smaller between clonally related tumors and larger between unrelated tumors, indicating the ability of the LOC or BGN tags to identify and distinguish between new and older clonal expansions (circles are averages of the fragment comparisons between the different xenografts, and bars are overall averages) D. Xenograft intragland PWDs are typically nearly as large as their intergland PWDs, indicating that the small tumor fragments are almost as diverse as their tumors.