Figure 1.

Analysis of blood sample using FISH based on microfluidic chip. Blood samples introduced into the microfluidic chip by capillary force and cells were attached on the channel surface by heating the microfluidic chip. Then Proteinase K introduced into the chip to digest the cells, followed by increasing the temperature of microfluidic chip for few sec to denature the DNA, then fluorescence tagged DNA probes introduced into the chip. Complementary DNA probes hybridized with the target sequence and emit fluorescence.