Figure 2.

Effect of HSV infection on INSM1 promoter-directed luciferase activities. (A) Schematic representation of the INSM1 promoter region from position -441 to +26. Predicted transcription factor binding sites are as follows: E (E box, 5′-CATTTG-3′, 5′-CACGTG-3′, and 5′-CATCTG-3′), S (SP1-binding site, 5′-GGGGCCGGGC-3′ and 5′-CGGGCGGGC-3′), and I (INSM1-binding site, 5′-CTCCAGGGGAAGC-3′). (B and C) Luciferase reporter assays. HaCaT (B) and HEp-2 (C) cells were infected with WT HSV-1 and UV-inactivated HSV-1 (MOI = 3) and harvested 9 h after infection. Luciferase activity was measured as described in Methods. The results are shown as means ± standard deviation (SD).