(A) Western blots of whole cell or nuclear proteins. (B) Western blots of Phos-tag gel detecting phosphorylation. PBA, LGKO mice received i. p. injection of 4-phenylbutyrate (PBA) after weaning. Each lane represents an individual mouse. (C) Unconventional splicing of Xbp1 mRNA by RT-PCR assay. uXbp1, unspliced Xbp1; sXbp1, spliced Xbp1. (D) Comparison of HOMA-IR index in WT versus LGKO. *P< 0.05, n= 6. (E) Immunoblots of mouse liver proteins. Ct, injected with PBS; Gl, injected with glucose; In, injected with insulin; IR, insulin receptor; IRS1-tyr, phospho-tyrosine of IRS1; IRS1-ser, phosphoserine of IRS1.