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. Author manuscript; available in PMC: 2012 Jul 1.

Published in final edited form as: Hepatology. 2011 Jul;54(1):216–228. doi: 10.1002/hep.24360

(A) Flow cytometric analysis of liver NPC for donor/recipient phenotype. Hepatic NPC from B7-H1 KO and WT grafts 6 hr after LTx into CD45.1 recipients were analyzed. CD3⁺CD4⁺, CD3⁺CD8⁺ and NK1.1⁺CD3^- cells were analyzed for CD45.1 (recipient) expression. Numbers are absolute numbers in liver grafts. Data are representative of three independent experiments.

(B) T cell apoptosis was determined by flow cytometry in liver NPC. CD3⁺CD4⁺, CD3⁺CD8⁺ and NK1.1⁺CD3^- cells were analyzed for CD45.1 and Annexin V expression. Annexin V expression by host CD45.1⁺ CD8, but not CD4 or NK cells was reduced in KO grafts compared with WT grafts. No differences were detected in Annexin V expression by graft CD45.1^- CD4, CD8 or NK cells between KO and WT grafts. Data are representative of three independent experiments.

(A) Flow cytometric analysis of liver NPC for donor/recipient phenotype. Hepatic NPC from B7-H1 KO and WT grafts 6 hr after LTx into CD45.1 recipients were analyzed. CD3⁺CD4⁺, CD3⁺CD8⁺ and NK1.1⁺CD3^- cells were analyzed for CD45.1 (recipient) expression. Numbers are absolute numbers in liver grafts. Data are representative of three independent experiments.

(B) T cell apoptosis was determined by flow cytometry in liver NPC. CD3⁺CD4⁺, CD3⁺CD8⁺ and NK1.1⁺CD3^- cells were analyzed for CD45.1 and Annexin V expression. Annexin V expression by host CD45.1⁺ CD8, but not CD4 or NK cells was reduced in KO grafts compared with WT grafts. No differences were detected in Annexin V expression by graft CD45.1^- CD4, CD8 or NK cells between KO and WT grafts. Data are representative of three independent experiments.