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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Acta Physiol (Oxf). 2011 May 26;204(2):277–287. doi: 10.1111/j.1748-1716.2011.02273.x

Figure 1. cAMP can both stimulate and blunt apoptosis in S49 lymphoma cells.

Figure 1

Wild-type S49 cells were incubated with 100μM CPT-cAMP, 500ng Anti-Fas or the combination of CPT-cAMP and Anti-Fas for 6h and 24h and then assayed for apoptosis (Annexin-V binding; left panel) or caspase 3 activity (determined using a colorometric assay kit from EMD; right panel). The results shown are from 3 separate experiments. *p<0.05, ** p<0.01 compared to control (0h), ## p<0.01 compared to anti-Fas alone by t-test analysis. Note that CPT-cAMP blunts Annexin V binding and caspase 3 activity induced by anti-Fas treatment at 6 hr but this blunting is lost by 24h and that CPT-cAMP increases caspase 3 activity at 24 hr as a prelude to apoptosis (Zhang and Insel, 2004; Zhang et al, 2008b).