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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Mol Carcinog. 2011 Mar 22;50(12):972–980. doi: 10.1002/mc.20767

Figure 5.

Figure 5

Figure 5

Figure 5

Figure 5

Figure 5A. Basal p53 expression status of each cell line used in clonogenicity assays. HepG2 cells are p53-proficient, while HepG2-p53si and Hep3B cells are p53-deficient. Lysates from each cell type were collected and analyzed by SDS-PAGE and Western blot using anti-p53 antibody and anti-β-actin as a loading control.

Figure 5B. Representative examples of colony formation across various cell lines for each plasmid construct utilized- mock, WtHBx and MutHBx, respectively. HepG2 cells are p53-proficient and HepG2-p53si and Hep3B cell lines are p53-deficient. Cells were transfected with 1.25 μg either mock, WtHBx or MutHBx/mL.

Figure 5C. Enhanced growth suppressive effect of MutHBx colonies compared to WtHBx colonies in p53-proficient hepatocellular carcinoma cells (HepG2) only. Results shown represent mean values from three independent experiments and bars indicate standard error of the mean. *p < 0.05; **p < 0.01.

Figure 5D. Unique splayed morphology of apparently dormant HepG2 cells expressing MutHBx. Cell morphology images of liver cell colonies taken 2 weeks into clonogenicity assay when colony formation in mock and WtHBx-transfected cells was well underway.