Skip to main content
. Author manuscript; available in PMC: 2011 Jun 29.
Published in final edited form as: Nat Neurosci. 2011 May 29;14(7):848–856. doi: 10.1038/nn.2839

Figure 7. Pol II stalling mediates immediate transcription of several IEGs.

Figure 7

(a) Pre-mRNA levels of various rapid IEGs and delayed IEGs five minutes after TTX washout in neurons infected with scrambled, nelfa-shRNA or nelfe-shRNA producing lentiviruses. n = 3 biological trials; error bars represent S.E. of the mean. (b,c) NELF-A depletion blocks immediate transcription of many IEGs. mRNA levels of different IEGs in neurons infected with nelfa-shRNA- or Sc-shRNA-producing lentiviruses. Cells were harvested either fifteen (b) or forty-five (c) minutes after TTX-withdrawal. The qPCR data were normalized to GAPDH and are presented as times of the corresponding mRNA level in scrambled shRNA expressing neurons with TTX. n = 3; error bars represent S.E. of the mean. For all panels, *P < 0.05 and **P < 0.001.