Table 2. MitoQ does not restore decreases in mitochondrial respiratory chain activity.

	Control	Ethanol	MitoQ 5 mg/kg/d	MitoQ 5 mg/kg/d + Ethanol	MitoQ 25 mg/kg/d	MitoQ 25 mg/kg/d + Ethanol
Complex Ia	118.14 ± 5.68	65.79** ± 5.59	111.36 ± 3.87	78.56**± 4.91	105.83 ± 8.38	81.56*± 6.54
Complex IIb	1.09 ± 0.04	1.22 ± 0.04	1.00 ± 0.07	1.37*± 0.13	1.06 ± 0.02	1.31± 0.05
Complex II-IIIc	60.24 ± 3.41	22.94**± 1.88	57.94 ± 5.09	26.24**± 2.24	61.10 ± 7.29	26.00**± 3.73
Complex IVd	53.72 ± 1.79	30.35**± 3.79	56.20 ± 3.06	33.10**± 2.81	56.99 ± 1.40	32.02**± 3.15
Complex Ve (ATPase)	175.26±17.89	149.12±13.78	147.77±11.47	144.44±8.65	181.52±13.52	184.58±11.72
Citrate Synthasef	0.115 ± 0.004	0.138*± 0.005	0.114 ± 0.005	0.138*± 0.006	0.110 ± 0.005	0.134*± 0.004

^a(nmol NADH/min/mg protein);

^bnmol DCPIP/min/mg protein;

^cnmol Cyt.c (reduced)/min/mg protein;

^d(k/sec)/mg protein;

^enmol NADH/min/mg protein;

^fμmol/min/mg protein

Activities were measured as described in materials and methods. Data represent the mean ± SEM for 6 pairs of rats on an ethanol-containing or control diet and 5 pairs of rats fed ethanol and control diets supplemented with MitoQ at 5 and 25 mg/kg/d. Statistical comparison were performed using one-way ANOVA whereas * and ** represents p≤0.05 and p≤0.01 respectively between ethanol-fed animals and corresponding pair-fed controls.