FIG. 3.

Subcellular localization of Prdx6 in rat lung alveolar epithelial type II cells. (A) Immunofluorescence using antibodies to 3C9, a marker for lung lamellar body membranes, and Prdx6. The merged image shows colocalization for the two proteins, indicating the presence of Prdx6 in lung lamellar bodies. (B) Ultrastructural localization of Prdx6 using electron microscopic immunohistochemistry with 20 nm gold-coupled antibody. Arrows indicate gold grains of Prdx6 in lamellar bodies (LB) with a lesser concentration of grains in other organelles. The right panel is a control in the absence of primary antibody. Modified from Wu et al. (103).