FIG. 3.

Cell surface expression and T cell recognition of H-2K^k SCT molecules presenting different peptides in TAP-deficient cells. (A) TAP2-deficient CHO cells were transfected with either the K^k class I heavy chain alone or K^k SCTs encoding the epitopes SIINFEKL (K^b-restricted from ovalbumin), SDYEGRLI (K^k-restricted from influenza virus nucleoprotein), or IEGGWTGMI (K^k-restricted from influenza virus hemagglutinin), as indicated. Cells were stained with the anti-K^k conformation-dependent mAb 11-4.1. Staining profiles of stable polyclonal cell populations are shown. (B) Cells shown in (A) were used as target cells in a standard 5 h chromium release cytotoxicity assay with T cell lines derived from influenza virus infected H-2^k mice as effector cells. Target cells expressed SCTs presenting either the peptide IEGGWTGMI (KkHASCT) or SDYEGRLI (KkNPSCT) as indicated, and were incubated with T cells specific for nucleoprotein (NP T cells) or hemagglutinin (HA T cells).