FIG. 4.

Cell surface expression and T cell recognition of a novel H-2D^d SCT construct. (A) CHO cells were either untransfected (UN) or transfected with a D^d SCT encoding the HIV-derived epitope RGPGRAFVTI, as indicated. Cells were stained with the anti-D^d conformation-dependent mAb 34-5-8S. The staining profile of a stable polyclonal cell population is shown. (B) Cells shown in (A) expressing the SCT were used as APCs to stimulate the T cell hybridoma B4.2.3 specific for RGPGRAFVTI presented by D^d, and IL-2 production was measured using standard enzyme-linked immunosorbent assay. CHO cells expressing the D^d class I heavy chain alone were used as a control. A D^d SCT has not been reported previously. APCs, antigen-presenting cells; IL-2, interleukin-2.