FIG. 1.
Growth curves of S. cerevisiae BY4741 (▪, □) and of the deletion mutant Δhaa1 (▴, ▵) in MM4 medium (pH 4.0) (▪, ▴) or in this same basal medium supplemented with 50 mM acetic acid (pH 4.0) (□,▵). The arrows indicate the time of cultivation at which cell samples were harvested to compare their transcriptomes. Growth was followed by measuring culture OD600 nm and the concentration of viable cells was assessed as the number of colony-forming units/mL of cell culture (CFU mL−1). The growth curves shown are means of, at least, three independent growth curves that gave rise to the same growth pattern.