FIG. 3.

Comparison of the transcript levels, estimated by real-time RT-PCR, of PDR12, HSP30, HRK1, MSN4, and TPO2 genes produced in cells of the parental strain BY4741 (wt) or the Δhaa1 mutant during cultivation for 30 min in MM4 growth medium (at pH 4.0) (white bars) or in this same growth medium supplemented with 50 mM acetic acid (dark bars). ACT1 mRNA was used as internal control. For each gene the value registered in parental strain control cells (corresponding to cells cultivated in unsupplemented growth medium) were set as 1 and the induction ratios are relative to this control. mRNA values shown are representative of results obtained, at least, in two independent incubation experiments.