FIG. 5.

Venn diagram illustrating the crosstalk between the regulatory systems controlled by the weak acid-responsive transcription factors Haa1p, Rim101p, Msn2/Msn4p, and War1p in yeast response to acetic acid stress. The 112 genes found to be upregulated in response to acetic acid (50 mM) in the parental strain were clustered among the referred regulons, based on the existence of documented regulatory associations with the indicated transcription factors, according with the information available in the YEASTRACT database (www.yeastract.com) (Teixeira et al., 2006b). The number of genes in each cluster is indicated and the full or the partial list of genes belonging to each regulon is shown in the schematic representation below the diagram, emphasizing the genes specific of each regulon and the coregulated genes. In the case of the genes specifically dependent on Haa1p, 10 genes were selected from those listed in Tables 1 (indicated in gray background) and 2 (indicated in a white background). These genes exhibit the highest reduction in transcript level in Δhaa1 cells, compared to the parental strain.