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. 2010 Oct;14(5):587–601. doi: 10.1089/omi.2010.0048

FIG. 6.

FIG. 6.

Comparison of the susceptibility toward acetic acid of mutants devoid of the expression of genes whose transcription is activated in response to the acid in a Haa1p-dependent manner. Cells of the parental strain (wt) and of the indicated deletion mutants were grown until mid-exponential phase in liquid MM4 medium (at pH 4.0) and then used to inoculate the same basal medium either supplemented or not supplemented with 60 mM acetic acid (at pH 4.0; initial OD = 0.05 ± 0.005). Cells were batch cultured at 30°C and growth was monitored based on OD600 nm. Three levels of susceptibility phenotypes were considered based on the level of inhibition of the growth curves of the parental strain and of the different deletion mutants. The growth curves shown are representative of at least four independent experiments that gave essentially the same results.