Table 1.
List of Genes Whose Acetic Acid-Induced Transcriptional Activation Registered in Cells of S. cerevisiae BY4741 Is Abolished in the Absence of HAA1 Expression
| |
|
(mRNA wt )ac |
(mRNA Δhaa1 )ac |
(mRNA wt )ac |
|
Induced by |
|||
|---|---|---|---|---|---|---|---|---|---|
| Gene or ORF | Biological function | (mRNA wt)c | (mRNA Δhaa1)c | (mRNA Δhaa1)ac | Acetic acid susceptibility | Actld. | Prop. | Sorb. | Lact. |
| TPO2 | Plasma membrane polyamine transporter of the major facilitator superfamily | 14.9 | 1.3 | 18.7 | + | ||||
| YLR297w | Putative protein of unknown function | 11.3 | 1.2 | 10.5 | − | ||||
| STP3 | Zinc-finger protein of unknown function | 2.6 | −1.4 | 8.1 | + | ||||
| YRO2 | Putative protein of unknown function | 11.1 | 1.4 | 7.8 | − | ||||
| YAR029w | Unknown function | 3.7 | −1.1 | 7.6 | − | ||||
| TOS3 | Protein kinase required for phosphorylation and activation of Snf1p; glucose metabolism | 5.7 | 1.1 | 6.2 | + | ||||
| YIR035c | Unknown function | 5.5 | 1.2 | 6.2 | − | ||||
| YGP1 | Cell wall-related secretory glycoprotein | 3.0 | 1.1 | 4.8 | + | ||||
| PCL10 | Cyclin involved in regulation of activity of protein kinase Pho85p | 5.0 | 1.1 | 4.3 | + | ||||
| YPR127w | Unknown function | 3.3 | 1.0 | 4.1 | − | ||||
| DSD1 | D-serine dehydratase | 3.2 | −1.1 | 4.0 | − | ||||
| MSN4 | Transcription factor activated in stress conditions | 6.1 | 1.4 | 3.8 | − | ||||
| YJR096w | Putative xylose and arabinose reductase | 2.7 | −1.1 | 3.7 | − | ||||
| SPI1 | GPI-anchored cell wall protein; involved in weak acid resistance | 5.1 | 1.4 | 3.5 | − | ||||
| HOR2 | Glycerol phosphatase involved in glycerol biosynthesis; induced in response to stress | 4.8 | −1.4 | 3.4 | + | ||||
| YKR075c | Unknown function | 2.9 | 1.1 | 3.3 | − | ||||
| SUR2 | Sphinganine C4-hydroxylase involved in sphingolipid metabolism | 3.5 | 1.2 | 3.0 | ++ | ||||
| ICY1 | Unknown function | 2.7 | 1.0 | 3.0 | + | ||||
| INM1 | Inositol monophosphatase involved in biosynthesis of inositol | 3.4 | 1.3 | 2.6 | + | ||||
| SAP30 | Subunit of a histone deacetylase complex | 3.2 | 1.3 | 2.6 | +++ | ||||
| YNL200c | Unknown function | 2 | −1.2 | 2.4 | ++ | ||||
| STF2 | Protein involved in the regulation of F1F0ATPase | 2.0 | −1.3 | 2.2 | − | ||||
| SYC1 | Subunit of the APT subcomplex of cleavage and polyadenylation factor | 2.9 | 1.1 | 2.2 | + | ||||
| YLR326w | Unknown function | 2.7 | 1.3 | 2.2 | − | ||||
| YAR028w | Unknown function | 4.0 | 1.1 | 2.1 | − | ||||
| YNL024c | Unknown function | 2 | 1.0 | 2.1 | − | ||||
| YNR034w-a | Unknown function | 2.2 | 1.3 | 2 | − | ||||
| GPG1 | Subunit of the heterotrimeric G protein that interacts with the receptor Grp1p; presumably involved in pseudohyphal growth | 2.2 | 1.1 | 2 | − | ||||
| PDE1 | Low afinitity cyclic AMP phosphodiesterase | 1.7 | 1.1 | 2.0 | − | ||||
| ADI1 | Acireductone dioxigenase involved in methionine salvage pathway | 1.9 | 1.0 | 2.0 | − | ||||
| YNL217w | Unknown function | 2.3 | 1.4 | 1.9 | − | ||||
| NRG1 | Transcriptional repressor involved in response to alkaline pH and glucose catabolic repression | 2.2 | 1.4 | 1.9 | ++ | ||||
| YPL071c | Unknown function | 2.3 | 1.3 | 1.8 | − | ||||
| MUK1 | Unknown function | 2.1 | 1.2 | 1.8 | − | ||||
| PDR12 | Plasma membrane ABC transporter involved in efflux of benzoate and sorbate anions | 4.2 | 1.3 | 1.8 | − | ||||
| TMA10 | Unknown function | 2.8 | 1.4 | 1.7 | − | ||||
| GRX8 | Glutaredoxin enzyme | 2.0 | 1.2 | 1.7 | − | ||||
| PFK27 | 6-phosphofructo-2-kinase | 2.1 | −1.4 | 1.7 | − | ||||
| FKH2 | Transcription factor involved in the expression of G2/M phase genes | 2 | 1.0 | 1.7 | − | ||||
| EEB1 | Acyl-coenzymeA:ethanol O-acyltransferase; presumably involved in lipid metabolism | 2.2 | 1.0 | 1.6 | − | ||||
| YLR346c | Putative protein of unknown function | 1.9 | 1.4 | 1.6 | − | ||||
| QCR10 | Subunit of the ubiqunol-cytochrome c oxidoreductase complex which comprises part of the mitochondrial respiratory chain | 1.6 | −1.1 | 1.5 | − | ||||
| ATG8 | Component of autophagosomes and Cvt vesicles; involved in intracellular trafficking | 1.6 | −1.1 | 1.5 | − | ||||
| YER188w | Unknown function | 1.7 | 1.4 | 1.5 | − | ||||
This table was prepared based on the comparative analysis of the transcriptome of cells of the parental strain BY4741 (wt) and of the mutant BY4741_Δhaa1 (Δhaa1) cultivated in MM4 medium (pH 4.0) supplemented with 50 mM acetic acid. The magnitude of the transcriptional activation registered in response to acetic acid stress in the parental strain (wtac/wtc) is shown as well as the susceptibility toward acetic acid of mutants devoid of Haa1p-target genes (−susceptibility identical to the parental strain;+,++,+++−increasing levels of susceptibility to the acid). The activation of Haa1p-target genes transcription in response to acetaldehyde (Actld), propionic acid (Prop), sorbic acid (Sorb), and lactic acid (Lact) is also indicated (shaded in gray) based on results reported from Aranda and del Olmo (2004), Mira et al. (2009), Schuller et al. (2004), and Abbot et al. (2008), respectively. Genes are sorted based on the ratio of mRNA gene level between the wild-type and the Δhaa1 mutant.