Table 2.
Linearity and Dilution
| Expected Concentration | Observed Concentration | %Recovery | |
|---|---|---|---|
| Intact FGF-23 (pg/mL) | |||
| Sample 1 | 69 | 69 | 100 |
| 35 | 40 | 114 | |
| 18 | 21 | 116 | |
| 9 | 12 | 133 | |
| 5 | 10 | 200 | |
| Sample 2 | 96 | 96 | 100 |
| 48 | 49 | 102 | |
| 24 | 37 | 154 | |
| 12 | 19 | 158 | |
| 6 | 10 | 167 | |
| C-terminal FGF-23 (RLU/mL) | |||
| Sample 1 | 97.2 | 97.2 | 100 |
| 48.4 | 48.48 | 93 | |
| 24.2 | 24.4 | 101 | |
| 12.2 | 12.0 | 98 | |
| 6.0 | 6.6 | 110 | |
| 3.0 | 2.0 | 67 | |
| Sample 2 | 182.4 | 182.4 | 100 |
| 90.2 | 90.8 | 100 | |
| 55.6 | 55.4 | 98 | |
| 37.8 | 37.2 | 98 | |
| 19.0 | 19.2 | 101 | |
| 9.4 | 8.8 | 94 | |
Samples were diluted using zero standard (serum matrix) and linearity of the intact and C-terminal FGF-23 assays was determined.