Fig.1.
C. elegans G-protein coupled receptor OCTR-1 is involved in immunity to P. aeruginosa. (A) Wild-type and octr-1(ok371) animals were exposed to P. aeruginosa. (B) Wild-type and octr-1(ok371) animals were exposed to heat-killed P. aeruginosa. (C) Wild-type and octr-1(ok371) animals were exposed to a full lawn of P. aeruginosa. The survival graphs represent combined results of two independent experiments, N=90 adult animals per strain. (D) Animals were placed on a small spot of P. aeruginosa in a 3.5cm plate and monitored over time for their presence or absence on the lawn. The graph represents combined results of three independent experiments, N=60 adult animals per strain. (E) Wild-type and octr-1(ok371) animals were exposed to P. aeruginosa expressing GFP for 48 hours and then visualized using a MZ FLIII Leica stereomicroscope. (F) Wild-type and octr-1(ok371) animals were exposed to P. aeruginosa expressing GFP for 48 hours and the colony forming units were quantified. Ten animals were used for each condition. The graph represents combined results of three independent experiments. Bars represent mean ± SEM.