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Comparison of photochemically and spontaneously evoked voltage changes recorded from a pyramidal neuron in a living brain slice. (a) Changes in membrane potential evoked by two-photon uncaging of MNI-Glu (bath application at 3 mM) at 720 nm. (b) Example of a single spontaneous miniature excitatory postsynaptic potential (EPSP). (c) Average of many EPSPs. Recordings were made at 23−25 °C from a layer 5 pyramidal neuron in an acutely isolated mouse brain slice.
