Figure 4.

Functional map of AMPA receptor current response from a hippocampal CA1 pyramidal neuron in living brain slice evoked by two-photon uncaging of CDNI-Glu. Upper panel, fluorescent image of a whole cell patch-clamped CA1 neuron filled with Alexa-594 imaged at 950 nm. Lower panel, each pixel was irradiated for 1 ms with mode-locked Ti:sapphire laser at 720 nm and 7 mW, and the evoked postsynaptic current was recorded. The current is shown on a pseudocolor scale. CDNI-Glu was applied from a puffer pipet just above the brain slice at 0.5 mM.