Fig. 3.

Uptake of MAC by phagocytosis and macropinocytosis. (A) Percentage of total MAC strain 104-infected macrophages containing punctate bacteria or large circles with bacteria inside following bacterial incubation in the indicated solution. (B) Raw 264.7 macrophages were infected by rhodamine-labeled MAC incubated in Middlebrook 7H9 broth prior to infection in the presence of rhodamine. (C) Bacteria incubated in the 24-h elemental mixture before rhodamine staining. (D to F) Bacteria incubated in elemental mixture D (D), elemental mixture E (E), and manganese chloride (F). The experiment was repeated three times. The white arrows point to evidence of bacterial entry by phagocytosis; the white arrowheads point to evidence of macropinocytosis.