Table 7.
Macrophage death after infection by MAC exhibiting the intracellular phenotypea
| Inoculum | % dead macrophages (MOI of 1,000/MOI of 100) | % cells stained by TUNEL assay (MOI of 1,000/MOI of 100) |
|---|---|---|
| Middlebrook 7H9 broth (control) | 4.02 ± 1.6/4.3 ± 0.6 | 12.3 ± 5.0/13.5 ± 2 |
| 24-h elemental mixture (control) | 5.04 ± 2.2/4.8 ± 1.9 | 15.8 ± 2.1/12.6 ± 3 |
| MAC from Middlebrook 7H9 broth | 8.58 ± 4.4/8.2 ± 2.5 | 11.0 ± 2.1/10.6 ± 2.7 |
| MAC from 24-h elemental mixture | 19.52 ± 4.9b/20.1 ± 3.7b | 19.8 ± 6.8/19.4 ± 4.9b |
a
Two hours postinfection, Raw 264.7 cells infected by MAC from the 24-h elemental mixture undergo cell death and apoptosis (measured by the TUNEL assay) more frequently than Raw 264.7 cells infected by MAC from Middlebrook 7H9 broth (control). The assay was repeated three times.
b
These values were significantly different (P < 0.05) from the value for MAC incubated in Middlebrook 7H9 broth.