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Schematic representation of the mutated positions in the nucleotide binding sites (NBS) of SecA2. The main secA2 gene coding regions are represented by NBS-I and -II (which bind ATP), PPB (which defines a putative binding region of SecA2-dependent proteins), and CD (which is a conserved domain of SecA proteins). The nucleotides modified by site-directed mutagenesis and the corresponding amino acids changes are indicated on the detailed NBS-I and -II sequences.
