Skip to main content
. 2011 Jun;79(6):2396–2403. doi: 10.1128/IAI.00020-11

Fig. 2.

Fig. 2.

Phenotypic and functional characterization of the NBS-SecA2 mutant of L. monocytogenes. (A) Morphology of wt, Del-SecA2, and NBS-SecA2 L. monocytogenes colonies grown on BHI agar plates at 37°C O/N as seen by light microscopy (magnification, ×10). wt bacteria exhibit smooth edges, whereas both SecA2 mutants (Del-SecA2 and NBS-SecA2) have craggy/rough edges. (B) Electron micrographs of wt, Del-SecA2, and NBS-SecA2 L. monocytogenes cultured at log phase. Left and right panels show low- and high-magnification views, respectively, of mutants and wt bacteria. Both mutants exhibit long filaments formed by multiple bacterial units, in contrast to individualized bacteria formed by wt L. monocytogenes. (C) SDS-PAGE analysis of TCA-concentrated stationary-phase culture supernatants from O/N-grown NBS-SecA2, Del-SecA2, and wt L. monocytogenes. Proteins were visualized by immunoblotting using a Listeria-specific antiserum.