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Activation of NF-κB in spleens from mice infected with the wild-type strain or the fnbA mutant strain. BALB/c mice were infected with SH1000 or JS1078. At 5 h after infection, spleens were removed and the spleen cells were harvested. Activation of NF-κB in the spleen cells was studied by electrophoretic mobility shift assay (EMSA). NSP, nuclear extract from spleen cells of SH1000-infected mice with a 200-fold molar excess of unlabeled dsDNA oligonucleotide.
