Table 2.
Primers used in this study
| Primer | Nucleotide sequence (5′ to 3′)a | Restriction site |
|---|---|---|
| cps.control.fw | GGCAGACAGAGTTAGGAGTTGAG | |
| cps.control.rev | CCCAGTCTTCGCCAGCAGC | |
| Kan.1 | GTGAATTGGAGTTCGTCTTG | |
| Kan.2 | TATGGACAGTTGCGGATGTA | |
| bgaA-1 | GCTCTAGACAAATCGTTGAACGAGGTGG | XbaI |
| bgaA-4 | GAAGATCTGTAATTTGATAGTCTTGACG | BglII |
| SPD1382for | GCACCTGCTTGCCAGAATAG | |
| SPD1382rev | AGTTATCGGCATAATCGTTATCGGATGTCAGCATATCAAT | |
| SPD1384for | GAGATCTAATCGATGCATGCCGGAATGACGACACTATCAC | |
| SPD1384rev | GCACCAAGAGGATCAATGAC | |
| eryrev | TAACGATTATGCCGATAACT | |
| eryfor | GCATGCATCGATTAGATCTC | |
| specfor | GTGAGGAGGATATATTTGAATACA | |
| specrev | AGTGTTTCCACCATTTTTTCAATT | |
| mgtAstart | CATGCCATGGCAGAAAGAGAGTCTGTTTTGC | NcoI |
| mgtAstop | TCTAGACCTTAAATCGCATTCTTG | XbaI |
| pmgtAlacZ5 | CGGGATCCTCCTCCTTGACATTGTGTGC | BamHI |
| pmgtAlacZ3 | GCTCTAGACTTGAGATTTGAGATAGATTGCTTC | XbaI |
| mgtAhisrev | CTAGTCTAGACCTTAATGGTGATGGTGATGGTGAATCGCATTCTTG | XbaI |
| controldmgtA.fw | CCTTGAACTAACTGTCCATACC | |
| controldmgtA.rev | CTCGATGAAGATATCATAGGC | |
| spr1740fw | GGAGAAAAGGGGGTGCTCTTGC | |
| spr1740.rev | TGTATTCAAATATATCCTCCTCACCTTCTACCTCCTAATAAGTTCC | |
| spr1738fw | AATTGAAAAAATGGTGGAAACACTGAGAGGAGAATGCTTGCGACA | |
| spr1738.rev | GACAAAGTCAGCTCAAATTACTG | |
| controldplyfw | GCTACTAAATTGACAGCGCGCC | |
| controldplyrev | CCTGCTAAGATGGTCTTGCCTTTC |
a
Restriction sites are underlined.