Western blot analysis of DS cultures for Spo0A, SigF, and SigG production by wild-type F5603, the agrB null mutant, and the complementing strain. Cells from 5-h DS cultures were collected and lysed. Those lysates were then Western blotted for Spo0A expression (A), SigF expression (B), and SigG expression (C). The molecular weight of each band is indicated on the left.