Table 2.
Primer | Construct or use | Sequence |
---|---|---|
mtrCfor1 | pMEB144 | CAGTTTGCCCAATCGTGGCGATAA |
mtrCrev1 | pMEB144 | ACCGCTCCAGCAAGTGTTAAGGAA |
H1P1 mtrC | pMEB191 | CATCAGTAAATAAACTGCTTATTGCATAAAGTGTAGCTTGAAAAATTATGATTCCGGGGATCCGTCGACC |
H2P2 mtrC | pMEB191 | AAATATCTGTGAACATGAAAATGCCTCAATTATAAGTTTGTTTCTATGGCTGTAGGCTGGAGCTGCTTCG |
glmUfor1 | qRT-PCR | TATGGGCACGGCGGTGACTTATTA |
glmUrev1 | qRT-PCR | TTTGCTGCGATAAGGCGGGTTAAG |
mtrCcompfor1 | pMEB219 | TATCGGCTTGTGCAATCG |
mtrCcomprev1 | pMEB219 | AGGTAGTTGGATATGTACAAGATT |
mtrCfor2 | Class conservation | TGTGGCATGCTCATAATTGATTT |
mtrCrev2 | Class conservation | CTAATCGCTAATACTGGGCGCT |
HD0281for1 | qRT-PCR | ATATCAGCTATGGCTGCTCCTCCA |
HD0281rev1 | qRT-PCR | CCGCTGCAGTTTGCCCTAAATCAA |
HD0769for1 | qRT-PCR | ATTACAACAATGGCTCAGCAGCCG |
HD0769rev1 | qRT-PCR | ACCGCCTTCATTAGACCAAGTCCA |
HD1155for1 | qRT-PCR | AGCTAGAGCGGCTGACCCATTAAA |
HD1155rev1 | qRT-PCR | GTGAGAAATTGCTCCGCTTTGGCT |
HD1435for1 | qRT-PCR | TCGGCCTCAGCAGTTACGCTTTAT |
HD1435rev1 | qRT-PCR | ACCCGCATTACGTATCGCATGTTC |