Bacterial strains |
|
|
E. coli DH5α |
F− φ80dlacZΔM15Δ(lacZYA-argF)U169 endA1 recA1 hadR17 (rK− mK−) deoR thi-1 supE44 gyrA96 relA1
|
Life Technologies |
C. perfringens
|
|
|
JIR325 |
Rifr Nalr derivative of strain 13 |
32 |
JIR12233 |
JIR325ΔrevRΩ erm(Q) |
This study |
Plasmids |
|
|
pT7Blue-3 |
Commercial cloning vector; Apr
|
Novagen |
pJIR750 |
E. coli-C. perfringens shuttle vector, carries pIP404 replication origin; Cmr
|
5 |
pJIR2715 |
Suicide vector base for C. perfringens; Cmr Ermr
|
6 |
pJIR3374 |
pJIR2715 (Asp718/BamHI)Ω JRP3520/JRP3521 (Asp718/BamHI, 1.8 kb) PCR product, Cmr Ermr
|
This study |
pJIR3429 |
pJIR3474 (XhoI/NotI)Ω JRP3718/JRP3719 (XhoI/NotI, 1.2 kb) PCR product, Cmr Ermr (revR suicide vector) |
This study |
pJIR3534 |
pJIR750 (BamHI/Asp718)Ω JRP3954/JRP3966 (BamHI/Asp718, 1.2 kb, Cmr (revR+ complementation plasmid) |
This study |